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Fig. S4. Effect of the overexpression of non-inducible forms of Ptf1a, Neurog2, NeuroD, or Ascl1 on Prdm13. (A-F) Dorsal views of neurula embryos injected unilaterally with the indicated mRNA and hybrized with a Prdm13 antisense probe. The pan-neuronal marker N-tubulin was used as a control. (I-L) Lateral views of the injected side of tailbud stage embryos misexpressing Ascl1 and hybridized with a Prdm13 antisense probe. N-tubulin, TH and Gad1 were used as controls. In all cases, LacZ mRNA was used as a lineage tracer. Note that Ptf1a and Ascl1 induce induce ectopic Prdm13 expression in the ectoderm and that Neurog2 and NeuroD reduce its expression (Ptf1a: 98% induced, n = 40 for Prdm13 and 93% induced, n = 28 for N-tubulin; Neurog2: 90 % reduced, n = 39 for Prdm13 and 100% induced, n = 27 for N-tubulin; NeuroD: 81% reduced, n = 38 for Prdm13 and 100% reduced, n = 26 for N-tubulin; Ascl1: 76 % induced, n = 38 for Prdm13; 94 %, n = 48 for N-tubulin; 100 % increased, n = 38 for TH; 100 % increased, n = 80 for Gad1). IS, injected side Image published in: Hanotel J et al. (2014) Copyright © 2014. Image reproduced with permission of the Publisher, Elsevier B. V. Experiment + Assay Source Phenotypes and Disease Xla Wt + ptf1a + neurula stage (in situ hybridization) A Expression Phenotype mislocalised prdm13.L expression in neural plate increased amount prdm13.L expression in neural tube Xla Wt + ptf1a + neurula stage (in situ hybridization) B Expression Phenotype increased amount tubb2b.S expression in ectoderm mislocalised tubb2b.S expression in ectoderm increased amount tubb2b.S expression in neural plate mislocalised tubb2b.S expression in neural plate Xla Wt + neurog2 + neurula stage (in situ hybridization) C Expression Phenotype decreased amount prdm13.L expression in neural plate Xla Wt + neurog2 + neurula stage (in situ hybridization) D Expression Phenotype increased amount tubb2b.S expression in ectoderm mislocalised tubb2b.S expression in ectoderm Xla Wt + neurod1 + neurula stage (in situ hybridization) E Expression Phenotype decreased amount prdm13.L expression in neural fold Xla Wt + neurod1 + neurula stage (in situ hybridization) F Expression Phenotype increased amount tubb2b.S expression in ectoderm mislocalised tubb2b.S expression in ectoderm Xla Wt + ascl1 + neurula stage (in situ hybridization) G Expression Phenotype mislocalised prdm13.L expression in ectoderm increased amount prdm13.L expression in ectoderm Xla Wt + ascl1 + neurula stage (in situ hybridization) H Expression Phenotype increased amount tubb2b.S expression in ectoderm mislocalised tubb2b.S expression in ectoderm Xla Wt + ascl1 + neurula stage (in situ hybridization) I Expression Phenotype increased amount th.S expression in ectoderm Xla Wt + ascl1 + neurula stage (in situ hybridization) J Expression Phenotype increased amount gad1.1.L expression in ectoderm Larger Image Printer Friendly View

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