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Fig. S9. mPrdm13 overexpression is not sufficient to activate Pax2 and other GABAergic markers in animal cap explants and induces gastrulation defects in embryos. (A) Real-time RT-PCR analysis of the expression of Pax2, Gad1 and Lbx1 in animal caps derived from embryos injected with mPrdm13 mRNA at different doses as indicated and collected when sibling embryos reached stage 26. Note that Pax2, Lbx1 and Gad1 are not activated by mPrdm13 expression. (B-D) Lateral view of tailbud stage embryos injected at the two-cell stage in each blastomere with mPrm13 mRNA. Note that strong gastrulation defects already occur at the lowest dose used (50 pg). Image published in: Hanotel J et al. (2014) Copyright © 2014. Image reproduced with permission of the Publisher, Elsevier B. V. Experiment + Assay Source Phenotypes and Disease Xla Wt + Mmu.prdm13 + tailbud stage (morphology) C Anatomical Phenotype abnormal gastrulation Xla Wt + Mmu.prdm13 + tailbud stage (morphology) D Anatomical Phenotype abnormal gastrulation Xla Wt + Mmu.prdm13 + tailbud stage (morphology) E Anatomical Phenotype abnormal gastrulation Larger Image Printer Friendly View

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