Xenbase Image ID: 85250

Fig. 3. Selected examples of marker gene expression in the developing Xenopus pronephric kidney. Lateral views are shown anterior to the right. Transcripts were detected by wholemount in situ hybridization. (A) Pax-8, stage 32. Expression is detected in the emerging pronephric tubules (arrowheads) and at reduced levels in the pronephric duct. (B) Pax-2, stage 36. All epithelia of the pronephric kidney including the nephrostomes (arrowheads) are stained. (C) Delta-1, stage 30. Staining is confined to the developing pronephric tubule anlage. (D) Wnt-4, stage 32. Expression is largely restricted to the nephrostomes (arrowheads). (E) Iro-3, stage 36. Transcripts are present in the common tubule only. Anterior (arrowhead) and posterior (arrow) ends of the expression domain are indicated. (F) Sal-1, stage 38. Expression is found in the common tubule (arrowhead) and the anterior segment of the pronephric duct (arrow indicates posterior end of the expression domain). (G) WT-1, stage 30. Staining is evident in the pronephric capsule. (H) Msr, stage 34. Staining marks the developing vasculature. The major blood vessels in the region of the pronephric kidney are indicated. In situ hybridization and photography was performed as described elsewhere (Heller and Brändli, 1997, 1999). Abbreviations: ACV, anterior cardinal vein; CCV, common cardinal vein; D, pronephric duct; PC, pronephric capsule; PCV, posterior cardinal vein; PS, pronephric sinus; T, pronephric tubules. Image published in: Brändli AW (1999) Copyright © 1999. Reproduced with permission of the Publisher, University of the Basque Country Press. Gene Synonyms Species Stage(s) Tissue pax8.L pax-8, XPax-8, XPax8 X. laevis Throughout NF stage 32 pronephric tubule pronephric duct otic vesicle pronephric kidney pax8.L pax-8, XPax-8, XPax8 X. laevis Throughout NF stage 35 and 36 pronephric kidney pronephric nephrostome pronephric duct pronephric tubule eye hindbrain midbrain-hindbrain boundary spinal cord otic vesicle optic stalk dll1.L delta-1, Delta-1, delta1, x-delta, X-delta-1, Xdelta-1, Xdelta1, XDelta1 X. laevis Throughout NF stage 29 and 30 pronephric tubule pronephric kidney brain spinal cord head eye cranial ganglion pharyngeal region wnt4.L wnt-4, Xwnt-4, Xwnt4 X. laevis Throughout NF stage 32 pharyngeal region brain pronephric kidney pronephric tubule trigeminal ganglion proximal tubule irx3.L irx3b, MGC131103, xiro-3, xiro3 X. laevis Throughout NF stage 35 and 36 pronephric tubule proximal tubule head region otic vesicle brain pronephric kidney sall1.S LOC108715370, sal-1, sal-like 1, sal1, sall1-a, sall1-b, sall1b, spalt-like 1, tbs, znf794 X. laevis Throughout NF stage 37 and 38 pronephric tubule spinal cord brain hindbrain eye early distal tubule pronephric kidney wt1.S wt-1, wt1-a, wt1-b, Xwt-1, xwt1 X. laevis Throughout NF stage 29 and 30 pronephric kidney glomus aplnr.L agtrl1, angio1, apj, apjr, aplnr-a, aplnr-b, hg11, msr, X-msr, Xangio1, Xmsr X. laevis Throughout NF stage 33 and 34 posterior cardinal vein anterior cardinal vein duct of Cuvier intersomitic vein aortic arch ophthalmic vein omphalomesenteric vein pronephric sinus Image source: Published Larger Image Printer Friendly View

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