XB-IMG-85255
Xenbase Image ID: 85255
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Fig. 5. Further analysis of noggin injected animal caps. (A) Stage 24 embryos stained with the antl-HNK-1 monoclonal antibody which
marks primary neurons. Anterior is at the top of the panel. (B) At the same early neurula stage, noggin injected animal caps are negative
for the anti-HNK-1 epitope. (C) Uninjected animal caps are also negative with the anti-HNK-1 antibody. (D) Whole embryos at the late
tailbud stage stained with the monoclonal antibody 6F11 that recognizes an NCAM antigen and marks neural tissue. (E) Noggin injected
animal caps react positively with 6F11 across most if not all of the animal cap showing that noggin injection results in the animal cap
acquiring a neural fate. (F) Uninjected animal caps are negative with the 6F11 antibody. (G,H,I) Sections through the samples shown in
O,E and F respectively. 6F11 is seen throughout the injected animal cap explant but is strongest in the superficial cells, though this may be
due to difficulties in antibody penetration in the whole-mount sample. There is little difference in the morphology of the animal caps in noggin
injected (H) and uninjected (!) animal caps. (J) Late tailbud embryos stained with the somite marker 12/101. (K) Noggin injected animal caps
do not express the antigen recognized by 12/101 confirming that noggin can neuralize animal caps without inducing the formation of
mesoderm (LI Uninjected animal caps do not express the 12/101 antigen. Bars in A and for the other whole embryos, 1.3 mm; In B and the
other intact animal caps, 0.25 mm; in G, 0.33 mm and in H, 0.06 mm. Image published in: Goldstone K and Sharpe CR (1998) Copyright © 1998. Image reproduced with permission of the Publisher.
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