XB-IMG-85281
Xenbase Image ID: 85281
|
Fig. S2. Analysis by transgenic embryos of the effects of Ras-dva1 downregulation. (A-A2) Whereas no cement gland inhibition is revealed in control transgenic embryos expressing wild-type Ras-dva1 under the control of Xag2 promoter (proXag2-wtRas-dva1-proCA-DsRed transgene), the cement glands are inhibited in embryos expressing Xanf1 under the same promoter (proXag2-Xanf1-proCA-DsRed transgene). (A) Bright light, (A1) DsRed fluorescence, (A2) overlay of panels A and A1. (B�C4) Whereas no cement gland inhibition is seen in control embryo of transgenic line bearing proXag2-EGFP construct and transfected by proXag2-wtRas-dva1- proCA-DsRed (B�B4), the embryo of the same line but transfected by proXAG2-dnRas-dva1-proCA-DsRed construct has no cement gland (C�C4). (D) No inhibition of FoxG1 expression is seen in the early neurula embryos bearing the control transgene proXag2-wtRas-dva1-proCA-DsRed (a). In contrast, a decrease of FoxG1 expression is observed in embryos transfected with proXAG2-Xanf1-proCA-DsRed. Whole-mount in situ hybridization with probes to both FoxG1 and DsRed. (E) Transgenic tadpole bearing the control proXag2-wtRas-dva1-proCA-DsRed construct has normal sized telencephalon marked by FoxG1 expression (a). At the same time, a reduction of the telencephalon and FoxG1 expression is seen in embryo bearing proXAG2-Xanf1-proCA-DsRed construct (b). Transgenic tadpoles were selected by revealing DsRed fluorescence and hybridized in whole-mount with the probe to FoxG1. Image published in: Tereshina MB et al. (2014) © 2014. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |