XB-IMG-85309
Xenbase Image ID: 85309
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FIG. 2. GREUL1 induces XAG-1 and Xotx2 from naı�ve ectoderm.
A total of 1 ng, 500 pg, or 250 pg of GREUL1 was injected into the
prospective ectoderm of a one-cell Xenopus embryo. The injected
ectoderm and noninjected control ectoderm were explanted at the
blastula stage and aged until stage 20. (A) The explants and a whole
embryo were then analyzed by RT-PCR for NCAM, Krox20,
HoxB9, c-actin, and the loading control EF-1 . The RT indicates
the whole embryo processed without addition of reverse transcriptase.
(B�K) Explants and uninjected whole embryos (B,G), were
stained for Xotx2 (B�F) and XAG-1 (H�K), by in situ hybridization.
For Xotx2 (B�F), the bottom of the figure shows the number of
explants exhibiting patches of darkly staining cells/the total number
of explants analyzed. 1 ng and 500 pg were significantly
different from the control at P values of less than 0.05, using a
Williams corrected G-test for independence. Image published in: Borchers AG et al. (2002) Copyright © 2002. Image reproduced with permission of the Publisher, Elsevier B. V.
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