Xenbase Image ID: 85337

Fig. 5. The expression of RFX4 is regulated by RFX7 and Foxj1. (A) RFX4 rescued NTD in RFX7 morphants. The bar-graphs show the quantitative assessment of the injections. At least three independent experiments were performed. “n” indicates the number of injected embryos. (B) Five hundred picograms of Foxj1 RNA was injected into a dorsal blastomere of 4-cell stage embryos and the gene expression was examined at stage 14 by whole mount in situ hybridization. β-gal staining (red) indicates the injected side. The number of embryos examined is indicated. (C) Foxj1 RNA was injected into two dorsal blastomeres of 4-cell stage embryos and the gene expression was examined at stage 14 by RT-PCR. Image published in: Manojlovic Z et al. (2014) Copyright © 2014. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue rfx7.L LOC108712931, rfxdc2 X. laevis Throughout NF stage 14 neuroectoderm neural plate rfx4.S X. laevis Throughout NF stage 14 neuroectoderm neural plate Image source: Published Larger Image Printer Friendly View

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