XB-IMG-85344
Xenbase Image ID: 85344
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Figure 6 Transcription activator�like effector nuclease-mediated eGFP disruption in vertebrates. (A�C) Fluorescence microscopy
images of eGFP_B TALEN-injected zebrafish embryos. eGFP_B TALEN mRNAs (100 pg each) were injected at 1�4 cell stage
zebrafish embryos obtained by reciprocal crossing of Tg(fli1a:eGFP) and Tg(flk1:mRFP). A merged image is shown in panel a,
eGFP fluorescence is shown in panel b, and mRFP fluorescence is shown in panel (C). The eGFP expression in a small population
of endothelial cells indicated by arrows was specifically suppressed, presumably due to the disruption of eGFP locus by the TALEN.
Similar mosaic eGFP expression was observed in eight independent embryos. (D) Cel-I assay of Mock or TALEN-injected
zebrafish embryos. Arrowheads indicate Cel-I-digested fragments. (E) Bright field and fluorescence microscopy images of eGFP_B
TALEN-injected frog embryos. (F) Cel-I assay of TALEN-injected frog embryos. Arrowheads indicate Cel-I digested fragments.
Right: A tadpole injected 600 pg right eGFP_B TALEN mRNA. Left and Right: A tadpole injected each 300 pg of left and right
eGFP_B TALENs mRNA. Image published in: Sakuma T et al. (2013) Copyright © 2013. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View |