Xenbase Image ID: 86632

Fig. 8. Impact of otogelin-like knockdown on the epidermis. (A) The otogelin-like gene is predicted to have up to 79 exons and 78 introns. An antisense morpholino oligo (red) was designed against the splice junction of exon 1 and intron 1. Loss of otogelin-like transcript is evident with otogelin-like MO compared with controls (MOC) by RT-PCR, indicating knockdown of expression. There is little difference in the intensity of bands for the ubiquitously expressed gene, ornithine decarboxylase (ODC). (B) Comparison of PNA staining in otogelin-like morphants compared with controls (MOC). Right panels are higher magnification images of the flank epidermis. (C) TEM images of goblet cells and SSCs in controls (MOC) and otog-like MO treated embryos. Note absence of dark core in the vesicles of the SSCs. Scale bars: in B, 250 μm (left panels) and 50 μm (right panels); in C, 1 μm. Image published in: Dubaissi E et al. (2014) © 2014. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

Return to previous page