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Fig. S5. Foxa1-HA misexpression. (A) Representative images showing misexpression of 10 pg HA-tagged foxa1 mRNA (and lineage tracer β-gal mRNA) in one side of the embryo and probed by in situ hybridization for SSC marker, otog-like, at stage 33. Chart shows comparison of mean number of SSCs in a defined area of the flank epidermis (otog-like positive) on injected and uninjected sides (n=9 embryos). Error bars represent s.e.m. Student�s t-test, P<0.05 (*). Scale bars: 500 μm. (B) Representative image showing embryo at stage 33 injected with higher dose (100 pg) of foxa1-HA mRNA and lineage tracer, membrane GFP (mGFP, green). PNA (red) is largely absent from areas where mGFP and foxa1-HA are misexpressed. The mGFP cells are numerous and form �masses�. Right panel is a higher magnification image of area enclosed in white box in the middle panel. Scale bars: 200 μm (left and middle panel) and 30 μm (right panel). Image published in: Dubaissi E et al. (2014) © 2014. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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