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Fig. S6. Foxi1-HA misexpression increases ionocytes but does not affect survival. (A) Representative images of an uninjected control and an embryo injected at the one cell stage with foxi1-HA mRNA (100 pg) and β-gal mRNA. Embryos were fixed at stage 30 and probed for the ionocyte marker gene, atp6v1a. Chart shows comparison of mean number of atp6v1a positive cells in a defined area on the flank epidermis of controls and foxi1-HA overexpressed embryos (n=5 embryos each). (B) Chart showing survival rates of controls and foxi1-HA misexpressed embryos both with and without supplementing with the antibiotic, Gentamicin (10 μg/ml). Results of three independent experiments. Error bars represent s.e.m. Student�s t-test, P<0.001 (***). Scale bars: 500 μm Image published in: Dubaissi E et al. (2014) © 2014. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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