XB-IMG-86653
Xenbase Image ID: 86653
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Figure 3. Back-tracking of Foxj1-positive superficial mesoderm (SM) cells to dorsal and ventral blastomeres at the four-cell stage. Four-cell embryos were oriented to be viewed from the dorsal (a-j) or lateral (k-t) side and time-lapse videos were recorded at about 1 frame per every 2 min. Embryos were fixed at Stage 10.5 and processed for Foxj1 WMISH (a, b, k, l). Movies were edited to mark Foxj1-positive regions or individual cells and back-tracked to the four-cell stage: Stage 10.5 (c, m), Stage 9.5 (d, n), Stage 9�9.5 (e, o), Stage 7�8 (f, p), Stage 6�7 (g, q), Stage 5 (h, r), 32-cell stage (i, s), four-cell stage (j, t). (a�j) Dorsal view. Central and lateral regions of the SM containing Foxj1-positive cells were outlined (a, b) and followed back to the four-cell stage (j). Note that lateral signals moved out of sight in dorsal perspective, starting at Stage 8 (f). (k-t) Right-lateral view. Six cells were marked with white (ventral) or black (dorsal) asterisks, according to their origin at the four-cell stage. One cell which originated from the ventral right blastomere and was characterized by Foxj1 expression at Stage 10.5 is marked by a white asterisk outlined in red. Image published in: Tingler M et al. (2014) Copyright © 2014. Image reproduced with permission of the Publisher, John Wiley & Sons.
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