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FIGURE 8. Knockdown of Dhrs3 interferes with somitogenesis. Dhrs3 MO (30 ng) was injected at the two-cell stage, and the morphants were collected at stage 28 for immunostaining and whole mount in situ hybridization analysis. A-B′, immunostaining with 12/101 antibody indicated that in Dhrs3 morphants the somites showed a diffused pattern and a reduction in immunoreactivity. C-H′, expression of somite marker genes myoD, pax3, and fgf8 on the dorsal side of the embryos showed the segmented expression pattern of somite units in the control embryos (C′-E′, arrow) was not observed in Dhrs3 morphants (F′-H′). I, real time PCR indicated that expression level of myoD, pax3, and fgf8 at stage 21 was down-regulated in Dhrs3 morphants. Image published in: Kam RK et al. (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue myod1.S mf25, MyoD, myod1-a, myod1-b, myogenic factor 3, XMyoD, XtmyoD X. laevis Throughout NF stage 28 somite presomitic mesoderm pax3.S cdhs, hup2, pax-3, pax3-a, pax3-b, Waardenburg syndrome 1, ws1, xpax3 X. laevis Throughout NF stage 28 hatching gland notochord intersomitic region fgf8.L AIGF, FGF-8, fgf-8b, fgf8a, fgf8b, MGC79739, Xfgf-8, Xfgf8 X. laevis Throughout NF stage 28 pharyngeal arch tail bud paraxial mesoderm Image source: Published Larger Image Printer Friendly View

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