Xenbase Image ID: 86766

Fig. 3. XAWP1 is required for neural crest induction. (A-I) One blastomere of a four-cell stage embryo was injected in the dorso-animal region with Co MO (5 ng), AWP1 MO1 (5 ng for B,C,E,F; 2 ng for G,I), AWP1 MO2 (20 ng) and hAWP1 (2.5 pg) as indicated and then subjected to in situ hybridization against Slug or Sox10 at stage 16. The injected side was traced by lacZ staining. All embryos are shown in dorso-anterior view with posterior to the top. (J) Four-cell stage embryos were injected in the animal pole region with the indicated combination of noggin (100 pg), XWnt8-CSKA (100 pg), Co MO (40 ng), AWP1 MO1 (20 ng) and AWP1 MO2 (40 ng), and the animal caps were dissected at stage 9 and cultured to stage 16 for RT-PCR analysis. (K-P) Two blastomeres of a four-cell stage embryo were injected in the dorsal-marginal region with Co MO (10 ng) or AWP1 MO1 (10 ng) and subsequently subjected to in situ hybridization against Xbra at stage 10.5 or MyoD or Xnot at stage 14. Embryos are shown in vegetal view with dorsal to the top (K,L) or in dorsal view with anterior to the top (M-P). Image published in: Seo JH et al. (2013) Copyright © 2013. Reproduced with permission of the Publisher, University of the Basque Country Press. Gene Synonyms Species Stage(s) Tissue tbxt.S bra, brachyury, ntl, t, t-a, t-b, X-bra, Xbra, Xbrachyury X. laevis Throughout NF stage 11 mesoderm myod1.S mf25, MyoD, myod1-a, myod1-b, myogenic factor 3, XMyoD, XtmyoD X. laevis Throughout NF stage 14 paraxial mesoderm not.S flh, floating head, not-a, not-b, not1, noto, Xnot, Xnot-2, Xnot1, Xnot2, Znot X. laevis Throughout NF stage 14 notochord sox10.L X. laevis Throughout NF stage 16 cranial neural crest snai2.L slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 X. laevis Throughout NF stage 16 cranial neural crest Image source: Published Larger Image Printer Friendly View

Return to previous page