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Xenbase Image ID: 86773

Fig. 1. Increases in NF-M and peripherin mRNAs at 9 days postcrush demonstrated by RNase protection. Ten g of total RNA pooled from 12 eyes were hybridized simultaneously with 32P-labeled RNA probes against Xenopus NF-M and peripherin in one tube, and 3 g of RNA were hybridized with a probe to Elongation Factor 1- (EF1- ) in a separate tube (B,C,D). A: Probe hybridized against yeast RNA, demonstrating that the RNase digestion went to completion. B: RNA from naive, unoperated eyes. C: RNA from the contralateral control eye, 9 days after optic nerve crush. D: RNA from the operated eye, 9 days postcrush. After correcting for a slight increase (10%) in the expression of EF1- mRNA, the ratios of RNA expression between the operated and contralateral control eyes (D,C) were 1.3 for NF-M and 2.3 for peripherin.

Image published in: Gervasi C et al. (2003)

Copyright © 2003. Image reproduced with permission of the Publisher, John Wiley & Sons.

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