XB-IMG-86773
Xenbase Image ID: 86773
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Fig. 1. Increases in NF-M and peripherin mRNAs at 9 days postcrush
demonstrated by RNase protection. Ten g of total RNA pooled
from 12 eyes were hybridized simultaneously with 32P-labeled RNA
probes against Xenopus NF-M and peripherin in one tube, and 3 g of
RNA were hybridized with a probe to Elongation Factor 1- (EF1- ) in
a separate tube (B,C,D). A: Probe hybridized against yeast RNA,
demonstrating that the RNase digestion went to completion. B: RNA
from naive, unoperated eyes. C: RNA from the contralateral control
eye, 9 days after optic nerve crush. D: RNA from the operated eye, 9
days postcrush. After correcting for a slight increase (10%) in the
expression of EF1- mRNA, the ratios of RNA expression between the
operated and contralateral control eyes (D,C) were 1.3 for NF-M and
2.3 for peripherin. Image published in: Gervasi C et al. (2003) Copyright © 2003. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View |