Xenbase Image ID: 86903

Fig. 2. Depletion of Sp8 by MO and TALEN phenocopy ecl mutants. (A) Sp8 gene model and sequences of MO and TALEN target. Translation start site is marked in red. Sequences of MO, 5′ UTR, and TALEN binding sites are denoted in italic uppercase, lowercase, and underline, respectively. Domains of Sp, buttonhead box, and zinc finger are colored orange, blue, and green, respectively. (B–E) Phenotypes of sp8 TALEN-injected embryos. (F and G) Phenotypes of sp8 MO-, mRNA-, and TALEN-injected embryos. Number of injected embryos is shown in parentheses. (H) sp8 TALEN target amplicon and Cel1 target. (I) Cel I assay for non-homologous end joining events in- duced by sp8 TALEN. Red arrows indicate mismatch cleavages due to mutation at the target. L is the DNA size marker. (J) Regional injection of sp8 TALEN leads to loss of limbs. UC, uninjected control. Image published in: Chung HA et al. (2014) Copyright © 2014. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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