Xenbase Image ID: 86904

Fig. 3. Inner ear development is aberrant in ecl mutants. (A–J) H&E staining of wax-embedded WT and ecl embryos at stage 46/47. (A and F) Anterior, (B, C, G, and H) middle, and (D and I) posterior sections. (Scale bar, 100 μm.) (E and J) SM in WT and ecl, respectively. (Scale bar, 25 μm.) Black arrows and bar indicate CVG and the thickness of SM, respectively. (E′) Image position of E and J. Immunofluorescence for α-catenin (K–K′′ and N–N′′), Islet 1 (L–L′′ and O–O′′), and acetylated-α-tubulin (M–M′′ and P–P′′) in WT and mutants, respectively. (Scale bar, 50 μm.) (K′, L′ M′, N′, O′, and P′) Hoechst staining and (K′′, L′′, M′′, N′′, O′′, and P′′) merged images. (Q and T) Staining of actin in WT and ecl OVs at stage 46/47. (Scale bar, 100 μm.) (R, S, and U) Sensory hair cells of the saccule and the cristae in WT and mutant embryos. (Scale bar, 100 μm.) (R′, S′–S′′′, T′, and U′) Hair cells of the saccule and the cristae in WT and mutant OVs. (Scale bar, 25 μm.) Compare U′ and R′ for SM staining. Ut, utricle; Sa, saccule; Oc, otoconia; ED, endolymphatic duct; AC, anterior cristae; HC, horizontal cristae; PC, posterior cristae; La, lagena; and SM, saccular macula. Image published in: Chung HA et al. (2014) Copyright © 2014. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue ctnna1 alpha(E)-catenin, cap102, Catna1 X. tropicalis Sometime during NF stage 46 to NF stage 47 saccular macula vestibulocochlear ganglion otic vesicle isl1.S islet-1, islet1, Xisl-1, Xislet-1 X. laevis Sometime during NF stage 46 to NF stage 47 otic vesicle vestibulocochlear ganglion saccular macula tuba4b.L alpha tubulin, alpha-tubulin, tuba4, tuba4a X. laevis Sometime during NF stage 46 to NF stage 47 otic vesicle saccular macula vestibulocochlear ganglion Image source: Published Larger Image Printer Friendly View

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