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Fig. S3. Both sp8 ATGMO and sp8 SBMO phenocopy ecl mutation. When injected bilaterally at the two-cell stage, both a translation blocking MO (ATGMO) and a splice-blocking MO (SBMO) phenocopied ecl mutants. Sp8 SBMO is designed to bind the sp8 exon 1/intron 1 junction. Sp8 MO represents these two MOs. The gross external morphology of these MO-injected embryos was normal but showed otic dysmorphogenesis with various penetrance (A′–D′). Enlarged otic vesicles were devoid of otoconia, whereas relatively normal sized otic vesicles had reduced, scattered, or fused otoconia. These embryos also showed circular and ventral swimming behaviors compared with their uninjected control or standard control MO (Con MO)-injected embryos that exhibit linear and dorsal-up swimming patterns. Phenotypes are summarized in E. Image published in: Chung HA et al. (2014) Copyright © 2014. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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