Xenbase Image ID: 87052

Fig. 9. Proliferating cell nuclear antigen (PCNA) immunoreactivity in a stage 51 tadpole (A,B) and a juvenile frog (D). A illustrates PCNA immunoreactivity in cells of the central field (cf) of the spinal cord, whereas, for comparison, C shows a neighboring section that was labeled by using in situ hybridization for XIF3 mRNA, which was found in the central field (cf) as well as in ventral motoneurons (m). After metamorphosis, PCNA immunoreactivity greatly diminished (D). B shows PCNA immunoreactivity in the retinal ciliary margin (c) of a stage 51 tadpole (which can be compared to a neighboring section labeled by in situ hybridization for XIF3 mRNA in Fig. 5A,C). The tadpole was stained with anti-PCNA at a dilution of 1:5,000, whereas juvenile frog spinal cord was stained with antibody at a dilution of 1:1,000. p, Pigmented retinal epithelium; i, inner nuclear layer; r, retinal ganglion cell layer. Scale bars 5 50 mm in B, 150 mm in C (also applies to A), 300 mm in D. Image published in: Gervasi C et al. (2000) Copyright © 2000. Image reproduced with permission of the Publisher, John Wiley & Sons. Gene Synonyms Species Stage(s) Tissue pcna.L X. laevis Throughout NF stage 51 spinal cord roof plate central canal dorsal funiculus eye retina ciliary marginal zone retinal neural layer retinal inner nuclear layer prph.L nef4, peripherin, XIF3 X. laevis Throughout NF stage 51 spinal cord roof plate floor plate ventral funiculus central canal lateral funiculus dorsal funiculus spinal neuron motor neuron Image source: Published Larger Image Printer Friendly View

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