Xenbase Image ID: 87116

FIG. 2. beta-Catenin requirement differs for dorsoventral and anterior patterning. 6 ng of MO was injected at the four-cell stage either into two dorsal or into two ventral blastomeres. While injections into dorsal blastomeres caused ventralization, injection into ventral blastomeres caused ventralization in few cases. (B) 6 ng of MO was injected into two cells of each of the four quadrants of the embryo. Injection into dorsal but not ventral quadrants caused embryos to develop with reduced heads but did not prevent the formation of dorsoventral axes and tail structures. (C) Sibling embryos of those shown in B were analyzed by RT-PCR and compared for the degree of expression of dorsal marl, ers at the early gastrula stage (stage 10). Siamois and Xnr3 were unaffected when ventral animal or vegetal cells were targeted, but reduced after dorsal injections of MO. Xwnt8 was increased after dorsal MO injection. ODC, ornithine decarboxylase used as a control for the efficiency of RNA synthesis in each sample. (D} One batch of embryos was injected with 10 ng of MO at the two-, four-, or eight-cell stage, targeting the dorsal vegetal area. Embryos were analyzed at the early gastrula stage for dorsal markers siamois, Xnr3, and chordin and head markers Xhex and cerberus. ODC was used as control for the efficiency of RNA synthesis in each sample. Embryos injected with MO at the two-cell stage have both dorsal and head markers depleted to below 10% of the uninjected control level at the gastrula stage. Eight-cell stage injections result in head marker Xhex and cerberus depletion~ while siamois, chordin, and Xnr3 are partially expressed. Image published in: Heasman J et al. (2000) Copyright © 2000. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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