Figure 3. Truncations or mutations of the rhodopsin COOH-terminal domain cause the fusion proteins to partially delocalize to the RIS. Confocal micrographs of transgenic retinas expressing (A) GFP-CT44del25; (B) GFP-CT44del5; (C) GFP-CT44/P353S; and (D) GFP-CT44/P353L. Nuclei were stained with Hoescht 33342. Truncation of the distal 25 (A) or 5 amino acids (B) or mutation of the penultimate proline to either serine (C) or leucine (D) resulted in partial delocalization of the fusion proteins to the lateral plasma membrane, Golgi, and synaptic terminal. GFP (green) and Hoescht 33342 (blue). os, outer segment; is, inner segment; n, nucleus; and s, synaptic terminal. Bar, 5 μm.
Image published in: Tam BM et al. (2000)
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