Figure 2. MCI induces MCC differentiation(a,b) Confocal images of the skin in embryos injected at the two-cell stage with MT-MCI and mRFP RNA (b) or mRFP RNA alone (a), fixed at stage 28, stained with an acetylated tubulin antibody to label cilia (green). Asterisks in b denote unusually large cells. (c,d) Confocal images of the skin in embryos injected with MCI-HGR RNA along with mRFP RNA as a tracer treated with DEX at stage 11.5 (d), and then fixed at stage 28 and stained for cilia (green). (e–g) The outer epithelium from donor embryos injected with mRFP RNA (red) was transplanted onto host embryos injected with mGFP RNA (green) as illustrated in Fig. 1g. Host (f) and donor (g) embryos were also injected with MCI-HGR RNA. Embryos were treated with DEX at stage 11.5 and fixed at stage 28. Shown are confocal images of a control transplant (e), a transplant onto a host injected with MCI-HGR RNA (f), or a transplant from a donor injected with MCI-HGR RNA (g), after staining for cilia (blue). (h) Cell types in the skin were scored as MCCs (green with blue cilia staining), PSCs (green with no cilia staining), outer cells (red), or outer cells with cilia (red with blue cilia). Data is presented as an average of 10–15 fields (±s.d.) obtained from at least three transplants where the asterisks denote experimental values significantly different from control values (p<.005). Scale bars =20microns
Image published in: Stubbs JL et al. (2012)
Image downloaded from an Open Access article in PubMed Central. Image reproduced on Xenbase with permission of the publisher and the copyright holder.
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