Figure 5. Smad6 protein accumulates at the plasma membrane and in the nuclei of overexpressing cells. Embryos were injected at the two-cell stage with 200 pg of plasmid pCS2+Smad6-myc, fixed at stage 12 and immunostained with antimyc antibody (9E10) which was visualized using a fluoroscein-conjugated anti-mouse secondary antibody (green; B,E) followed by nuclear staining with propidium iodide (red; A,D). Overlap of nuclear and antimyc expression appears light green (C,F).
Image published in: Nakayama T et al. (1998)
Copyright © 1998. Image reproduced with permission of the Publisher, John Wiley & Sons.
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