Fig. 3. The effect of Xdsh mRNA depends on the site of injection. A single prospective dorsal (A) or ventral (B,D) vegetal blastomere of cleavage-stage embryos (8-16 cells) was injected with 0.4 ng of Xdsh mRNA. Phenotypes of the injected neurulae (A,B) and of tadpoles at stages 40-42 (C,D) are presented. Axis duplications are clearly visible in embryos injected with Xdsh RNA in a ventral blastomere (B,D). Embryos, injected ventrally with 0.4 ng of a control ÆXdsh mRNA (C), are indistinguishable from normal tadpoles or from the tadpoles, injected dorsally with Xdsh mRNA (as in A). Note that three embryos in D have completely duplicated body axes including most anterior and posterior structures, whereas in one embryo both dorsal axes oppose each other and posterior development is inhibited.
Image published in: Sokol SY et al. (1995)
Copyright © 1995. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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