Figure 3. Expression of Xwnt-8 and CAT transcripts following injection of CSKA-X8 or CSKA-CAT DNA into Xenopus embryos. (A) RNA was isolated from injected or uninjected embryos at cleavage (stage 3), blastula (stage 9), gastrula (stage 11 ), neurula (stage 21), or tailbud (stage 32) stages, as indicated at the top of A [(N/F) Nieuwkoop and Faber 1967] and subjected to gel blot analysis. The filter was successively hybridized with the probes indicated at left. (B) Gastrulae (stage 10; lanes 1-6) or neurulae (stage 21; lanes 7-14) were dissected as illustrated. Northern blots containing RNA from the region indicated above each lane were successively hybridized with the probes indicated at left. The filter was exposed to film for 10 hr, or for 6 hr, to obtain the Xwnt-8 signals shown for lanes 7-14, and 1-6, respectively. (D)Dorsal; (V) ventral; (W) whole; (H) head. (C) Whole-mount in situ hybridization of Xwnt-8 probes to stage-11 gastrulae that had received dorsal injections of CSKAX8. Solid arrows denote signal from endogenous Xwnt-8 transcripts in ventrolateral maginal zone ceils; open arrows indicate staining of dorsal lip cells from plasmid-derived Xwnt-8. (A) Whole gastrulae; (B) sagittal section through a representative embryo.
Image published in: Christian JL and Moon RT (1993)
Copyright © 1993. Image reproduced on with permission of the Publisher, Cold Spring Harbor Laboratory Press. This is an Open Access article.
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