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Figure 2. In situ hybridization analysis of developmental expression of the Xenopus alpha -MHC and vMHC genes. A: Lateral view of stage 26 embryos showing alpha -MHC transcripts in the bisymmetric heart patches. B: Ventral view of embryo in A. C: Transverse section through the heart patch of stage 26 embryo. D: Lateral view of stage 30 embryo showing increased alpha -MHC expression in precardiac tissues (arrow). E: Lateral view of stage 30 embryo showing an absence of vMHC expression at this stage of development. F: Lateral view of stage 38 embryo showing alpha -MHC expression in the heart, muscles of the jaw (jm), and the lymph heart (lh). G: Transverse section through stage 36 heart shows that alpha -MHC expression is confined to the myocardium. H: Transverse section through the lymph heart (stage 38) stained for alpha -MHC. I,J: Whole-mount and section in situ hybridizations, respectively, of stage 45 heart showing that alpha -MHC is expressed in all chambers and outflow tract. K,L: Whole-mount and section in situ hybridizations, respectively, of stage 45 heart, showing restricted expression of vMHC in a ring around the outflow tract and the atria at the junction with the ventricle. M: Section in situ of adult Xenopus heart shows that alpha -MHC is expressed throughout the myocardium, including the atria (a) and outflow tract. N: Section in situ hybridization of adult Xenopus heart shows that vMHC is expressed in the myocardium of the outflow tract and ventricle but not the atria.

Image published in: Garriock RJ et al. (2005)

Copyright © 2005. Image reproduced with permission of the Publisher, John Wiley & Sons.

GeneSynonymsSpeciesStage(s)Tissue
myh6.SaMHC, ck231, MHC, MHC-alpha, MHCa, MHCalpha, muz, muzak, XCMHCX. laevisThroughout NF stage 26heart primordium
cardiac mesoderm
myh6.SaMHC, ck231, MHC, MHC-alpha, MHCa, MHCalpha, muz, muzak, XCMHCX. laevisThroughout NF stage 37 and 38right lymph heart
left lymph heart
jaw muscle
heart
myh6.SaMHC, ck231, MHC, MHC-alpha, MHCa, MHCalpha, muz, muzak, XCMHCX. laevisThroughout NF stage 35 and 36myocardium
myh6.SaMHC, ck231, MHC, MHC-alpha, MHCa, MHCalpha, muz, muzak, XCMHCX. laevisThroughout NF stage 29 and 30myocardium
heart
myh15.Lmyh7, vMHCX. laevisThroughout NF stage 45left atrium
right atrium
heart
outflow tract
myh15.Lmyh7, vMHCX. laevisThroughout adult frog stagecardiac ventricle
outflow tract
heart
myh6.SaMHC, ck231, MHC, MHC-alpha, MHCa, MHCalpha, muz, muzak, XCMHCX. laevisThroughout NF stage 45heart
cardiac ventricle
left atrium
right atrium
cardiac mesoderm
outflow tract
myh6.SaMHC, ck231, MHC, MHC-alpha, MHCa, MHCalpha, muz, muzak, XCMHCX. laevisThroughout adult frog stagemyocardium
left atrium
right atrium
outflow tract

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XB-IMG-1354