Fig. 4. RARγ2 knockdown alters expression of posterior Hox and PSM markers. (A-J′) Embryos were injected unilaterally at the 2- or 4-cell stage with 7.5 ng Rarγ2.1 MO+7.5 ng Rarγ2.2 MO. Injected side is indicated by magenta β-gal lineage tracer. Rarγ2.1/2.2 MO decreases expression of (A) Hoxc10 (18/18 embryos), (B) Hoxd10 (12/12), (C) Hoxa11 (9/9) and (D) Hoxc13 (16/16) in tailbud stage embryos. Rarγ2.1/2.2 MO decreases lateral, but expands midline, expression (green lines) of (E) Msgn1 (10/13) and (F) Tbx6 (8/11), knocking down and shifting expression rostrally of (G) Thyl2 (13/15) and (H) Ripply2 (13/14) in tailbud stage embryos. Rarγ2.1/2.2 MO decreases lateral, but expands midline, expression (green lines) of (I) Msgn1 (35/36) and (J) Tbx6 (20/20) in neurula stage embryos. Embryos shown in dorsal view with anterior on left. (I′,J′) Caudal views of I and J.
Image published in: Janesick A et al. (2014)
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