Fig. 7. VP16-RARγ2 overexpression knocks down posterior Hox and PSM marker expression. Embryos injected unilaterally at 2- or 4-cell stage with 0.3 ng VP16-Rarγ2 mRNA or control (mCherry) mRNA. Injected side is indicated by magenta β-gal lineage tracer. Control expression of Hoxc10, Hoxd10, Msgn1 and Tbx6 is shown in Fig. 6A,C,G,I. (A-D) VP16-Rarγ2 overexpression shortens the axis on injected side in 100% of embryos. (A,B) VP16-Rarγ2 mRNA results in neural/midline rostral shift and lateral knockdown in Hoxc10 (9/13 embryos) and Hoxd10 (7/13) expression. Neural/midline knockdown is also observed (Hoxc10, 4/13; Hoxd10, 7/13). (C,D) VP16-Rarγ2 mRNA rostrally shifts and/or knocks down Msgn1 (12/12) and Tbx6 (13/13) expression. Tailbud embryos shown with anterior to left. (A′-D′) Caudal views of A-D.
Image published in: Janesick A et al. (2014)
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