Figure 4. Derepression of TH-response genes in hind limb phenotype tadpoles. Total RNA from whole bodies of F1 normal or hind limb (HL) phenotype tadpoles at NF stage 48–49 and NF stage 50–51, respectively, was isolated from four clutches [clutches 6, 7, 9, and 11 (Supplemental Table 1)] was isolated to analyze mRNA expression before TH circulation begins at NF stages 54–55. HL phenotype animals had higher expression levels of TRβ (A), ST3 (B), and KLF9 (C) mRNA than normal animals. D, The TALEN-induced mutation in TRα had no effect on its mRNA expression levels, which were not significantly different in normal and hind limb phenotype animals (n = 4–6); bars show expression levels relative to the housekeeping gene rpL8, and error bars represent SD. Significance levels for a one-way ANOVA were: *, P < .05; **, P < 0.01; ***, P < 0.001.
Image published in: Choi J et al. (2015)
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