Fig. 4. Analysis of individual offspring of F0 frogs. (A) Phenotypic analysis of the offspring obtained by mating two F0 frogs, m1 and f1. The percentages of wild-type and albino F1 tadpoles are indicated by black and white bars, respectively, and the values are denoted in the bars. The total number of tadpoles is shown on the right. (B) Genotypic analysis of individual offspring obtained from mating the m1 and f1 frogs. The target DNA fragment was amplified using genomic DNA that was purified from individual F1 tadpoles and was recloned for sequence determination. The phenotype (Ph) and genotype (Ge) of F1 tadpoles are indicated on the left. Each F1 tadpole had a pair of sequence lines as shown in brackets. wt, wild-type target sequence; in, in-frame mutation; out, out-of-frame mutation; L, large deletion containing the start codon or the exon-intron boundary. The ratio of the number of tadpoles with a pair of the indicated sequences to the total number of tadpoles with the same phenotype and genotype is shown in parentheses on the right. The alignment is labeled as described in the legend of Fig. 3F.
Image published in: Nakajima K and Yaoita Y (2015)
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