Fig. 2. Luminescence profiles of [Ca2+]i in embryos injected with aequorin into different locations at the 8-cell stage. (A) Aequorin injected into ventral micromeres is located in the ventral ectoderm during gastrulation. When injected into equatorial locations of either (B) ventral or (C) dorsal micromeres, it becomes located in the ventral and dorsal mesoderm, respectively. (D) Aequorin injected into the macromeres is located in the endoderm during gastrulation. In each panel the inset illustrates the location of the injection site (left) and the subsequent location of the aequorin during gastrulation (on the right; shaded zone). Under all these injection protocols, no significant [Ca2+]i increase was detected. AP, animal pole; VP, vegetal pole; V, ventral; D, dorsal.
Image published in: Leclerc C et al. (2000)
Copyright © 2000. Image reproduced with permission of the Publisher, The Company of Biologists Ltd.
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