Fig. 4. The changing pattern of localized [Ca2+]i transients that occur in the dorsal ectoderm during gastrulation. (A) Images of a representative embryo at stages 8-9, 10, 11, 12 and 13, on to which are superimposed yellow spots to mark the position of each localized Ca2+ transient observed throughout each stage. (B) Drawings reconstructed from the corresponding video images of A. (C) Drawings of animal-vegetal (upper) and sagittal (lower) sections of embryos at stages 9 and 10, respectively. Superimposing the images in A on the drawings in B illustrates the distribution of the positions of the localized calcium transients relative to the anteroposterior axis of the dorsal ectoderm and the blastopore lip observed at each developmental stage. The approximate extent of this distribution is illustrated by the red shaded region in B. AC, animal cap; NIMZ, noninvoluting marginal zone; IMZ, involuting marginal zone. Scale bar, 0.5 mm. (D) Histogram showing the amplitude of the transients versus developmental stages.
Image published in: Leclerc C et al. (2000)
Copyright © 2000. Image reproduced with permission of the Publisher, The Company of Biologists Ltd.
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