Figure 4. The V332A mutation abrogates peripherin’s outer segment targeting in rods of the rds mouse.(A) Wild type FLAG-tagged mouse peripherin (mPer) was electroporated into rods of the rds mouse and stained with anti-FLAG antibodies (green). (B) V332A FLAG-tagged peripherin electroporated into rods of the rds mouse; construct mislocalization to the inner segment and synaptic terminal is highlighted by white arrowheads. (C) V332A FLAG-tagged peripherin electroporated into the rods of wild type mice. The electroporated constructs are illustrated schematically above the corresponding panels with the position of the FLAG tag depicted in red. Note that the outer segment shapes in (A) and (C) are different due to ongoing photoreceptor degeneration in the rds mouse (A). Abbreviations are: OS – outer segment, IS – inner segment, N – nuclei, ST – synaptic termini. Nuclei (blue) are stained with Hoechst. Scale bar: 10 µm.
Image published in: Salinas RY et al. (2013)
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