Isoform Differential Expression in Clutch A, see also Fig S5 A – Isoform switching event examples: maternal to maternal; maternal to zygotic; and zygotic to zygotic switching events (left). Histogram of isoform switching events by category and time (right). B – bicd2 isoforms switch in PolyA+ data (circles), and do not switch in rdRNA data (squares) indicating differential polyadenylation. PolyA+ and rdRNA abundances agree within uncertainty of the absolute normalization (Supplemental Experimental Procedures). C – Normalized read depth on bicd2 locus in Clutch A polyA+ between 0–4.5 hpf. Note temporal dynamics shared by the final three bicd2(1) exons. Heatmap and depth of pile ups corrected for changing total mRNA. D – Spatial expression of three genes with isoforms with differential dynamics. All isoforms show different temporal and spatial expression domains. Grey lines and points mark total expression for each gene (sum of the red and blue isoforms).
Image published in: Owens ND et al. (2016)
Copyright © 2016. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| calhm2 | X. tropicalis | Throughout NF stage 3 (4-cell) | blastomere | |
| calhm2 | X. tropicalis | Throughout NF stage 33 and 34 | head pharyngeal region trigeminal nerve | |
| ckb | b-ck, ckbb, XCK1 | X. tropicalis | Throughout NF stage 9 | marginal zone |
| ckb | b-ck, ckbb, XCK1 | X. tropicalis | Sometime during NF stage 31 to NF stage 32 | eye somite pineal gland |
| btbd3 | X. tropicalis | Throughout NF stage 4 (8-cell) | blastomere | |
| btbd3 | X. tropicalis | Throughout NF stage 33 and 34 | central nervous system midbrain-hindbrain boundary spinal cord |
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