Transcript copy numbers and kinetics per embryo in Clutch A A – Transcript copy number histogram in polyA+ sequencing at 0.0 (egg), 4.5 hpf (early stage 9), 10 hpf (stage 12.5, end of gastrulation), 66 hpf (tadpole stage 42). Detection limits give number of transcripts required to generate a single read averaged over all transcript lengths (Fig S2E). Transcript copy number distributions transition smoothly between these timepoints (data not shown). B – Histogram of maximum rates of increase in transcripts/hour between any consecutive measurement points calculated from Gaussian process medians for each gene. Blue – PolyA+ 0–23.5 hpf; Red – rdRNA 0–23.5 hpf; Green – PolyA+ 24–66 hpf. Vertical lines mark the gene with max. rate of increase for each category. C – As B but kb/hour. D – Maximum rate of increase in transcripts/hour (left) and kb/hour (right) for 0–3.5 hpf and 4–23.5 hpf time intervals. PolyA+ and rdRNA distributions are discrepant for 0–3.5 hpf reflecting polyadenylation of maternal RNAs. Distributions are identical over 4–23.5 hpf.
Image published in: Owens ND et al. (2016)
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