Fig. 3. E-cadherin knockdown blocks CNC migration in vivo. (A) In situ hybridization of CoMO and E-cadMO injected embryos with CNC marker twist and AP2 demonstrates that E-cadherin knockdown causes CNC migration defect. The migration can be restored by co-injection of E-cadMO resistant full-length E-cadherin. (B) Statistic of the in situ hybridization. N=number of experiments and n=number of embryos. (C) Transplanted E-cadherin knockdown CNC cells were in contrast to mbGFP injected CNC cells unable to migrate ventrally into the branchial arches. (D) Statistic of the transplantation experiment. (E) E-cadherin knockdown in CNC cells leads to branchial specific cartilage defect. Ventral view of control morpholino (CoMO) and E-cadherin morpholino injected embryo after cartilage staining with Alcian Blue at stage 44 is shown. Black dashed line indicates the reduced gill cartilage on E-cadherin depleted side of the embryo. White dashed line shows the middle line of the entire cartilage structure. * Indicates injected site and bars show the mean percentage of embryos with CNC migration defect with standard deviations. Significance is calculated according to fisher’s exact test. Scale bar: 200 μm.
Image published in: Huang C et al. (2016)
Copyright © 2016. Image reproduced with permission of the Publisher, Elsevier B. V.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| twist1.S | twist, twist1-a, twist1-b, X-twi, Xtwi, Xtwist | X. laevis | Throughout NF stage 27 | neural crest cranial neural crest mandibular crest hyoid crest branchial arch |
| tfap2a.L | ap2, AP2a, AP2alpha, tfap2a-a, tfap2a-b, tfap2alpha, XAP-2, xap2 | X. laevis | Throughout NF stage 27 | neural crest cranial neural crest mandibular crest hyoid crest branchial arch periotic region head head mesenchyme pharyngeal arch |
Image source: Published
| Experiment + Assay | Source | Phenotypes and Disease | ||
|---|---|---|---|---|
| Xla Wt + cdh1 MO + NF25 (in situ hybridization) | fig.3. a, b |
| ||
| Xla Wt + cdh1 MO + NF25 (in situ hybridization) | fig.3. e |
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