Fig. 4. BMP pathway overactivation affects the formation of MCCs and mucus-producing cells in HAEC cultures. (A,A′) Chronic treatment of HAEC cultures with 100 ng/ml BMP2 drastically decreased the numbers of both MUC5ACpositive goblet cells and acetylated tubulinpositive MCCs relative to untreated controls. DAPI was added to confirm the presence of cells (A′). (B) Quantification of the experiments illustrated in A,A′ performed in triplicate; data show mean± s.d. from three donors; ***P<0.001, **P<0.01, Student’s t-test. (C) HAECs chronically exposed to 100 ng/ml BMP2 upregulated the squamous metaplasia markers involucrin (IVL) and transglutaminase 1 (TGM1). CTR, control; UBC, ubiquitin C.
Image published in: Cibois M et al. (2015)
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