Figure 3. Openers response by sulfonylurea receptor 1 (SUR1) and SUR2A wt and relative mutants. Residues Q1342, I1347, and L1350 of SUR1, and the matching residues E1305, I1310, and L1313 of SUR2A were mutated into alanine, glycine, and isoleucine. (A) The effects of the application of 100 μmol/L MgADP were measured in inside-out patches excised from oocytes coexpressing Kir6.2 and the indicated SUR constructs. Currents were normalized to the current measured in the absence of nucleotides immediately before MgADP application. Numbers at right of bars indicate the number of patches included in each average. (B) The effects of 300 μmol/L Diazoxide or 10 μmol/L P1075 were measured in inside-out patches excised from oocytes. Diazoxide (300 μmol/L) was applied in the presence of 100 μmol/L ATP. P1075 (10 μmol/L) was applied in the presence of 100 μmol/L ATP. Control designates the current measured in 100 μmol/L ATP before application of the tested opener.
Image published in: Principalli MA et al. (2015)
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