Fig. S3. MO-specificity, foxj1 expression and ciliogenesis in Htr3 morphants. (A-C) MO specificity. MO1 and MO2 specificity was tested using an eGFP-reporter construct. A 60 bp fragment of Htr3 including the translational start site as well as bindings sites for both MO1 and MO2 (cf. Fig. 1C) was cloned in frame with eGFP (cf. Gessert et al., 2010). Injection into animal blastomeres of 4-cell embryos were performed using rhodamine-B dextran as lineage tracer. (A) Co-injection of co-MO did not affect green fluorescence of reporter construct in targeted regions at stage 9. (B, C) Absence of green fluorescence upon co-injection of MO1 (B) or MO2 (C) demonstrated MO-specificity. (D, E) Unaltered foxj1 mRNA expression in the frog epidermis of Htr3 morphant. Embryos shown in lateral view, anterior to the left. (F, G) Wildtype and morphant epidermis at stage 34 stained for cilia (red) and actin (green) using anti-acetylated-α-tubulin antibodies and phalloidin. MCC number and ciliation were unaffected in morphants (F', G', F'', G''). Increasing magnifications. Scale bars represent 100μm (F, G), 50μm (F', G') and 10μm (F'', G'').
Image published in: Walentek P et al. (2014)
Copyright © 2014. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| foxj1.S | foxj1-a, foxj1-b, hfh-4, hfh4, xfoxj1 | X. laevis | Throughout NF stage 16 | epidermis neural tube |
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