Fig. 8. (A) Schematic of transcripts used for a3 ectopic overexpression experiments. hxa3 encodes a full length chimeric transcript containing the human a3 signal sequence. txa3 represents a truncated, non-translatable form of the a3 transcript. (B) Western blots of total protein extracts derived from stage 9 embryos injected with the hxa3 or txa3 transcripts. D3FAP recognizes low levels of endogenous a3 in uninjected (U) and txa3 injected embryos. In contrast, high levels of a3 are detected in hxa3 injected embryos. The 8C8 Western blot is included as a control to demonstrate equal loading of samples from injected and non-injected embryos.
Image published in: Meng F et al. (1997)
Copyright © 1997. Image reproduced with permission of the Publisher, Elsevier B. V.
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