Fig. 6. Mutations in predicted functional domains of Wbp2nl, indicated in Fig. 1A, affect its ability to change embryonic gene expression. (A) Percentages of embryos that show ectopic neural plate expression of indicated genes after injection of wild type (WT) or mutated mRNAs: T45A, Y55F and Y91G disrupt putative phosphorylation sites in the PH-G domain; F127P disrupts an α-helix in the PH-G domain; Y282F disrupts a putative YAP binding site at the C-terminus (see Fig. 1A). Sample sizes are numbers at the base of each bar. *, p < 0.05; **, p < 0.01; ***, p < 0.005, Chi-squared statistic. (B) Two examples of more intense and broader ectopic expression domains of foxd3 or zic2 in the neural plate (np) after expression of two mutant proteins (Y55F; Y91G). Compare to WT images (Fig. 3C, D). Dorsal views, anterior to the top. (C) Percentages of embryos that show reduced neural plate expression of indicated genes after expression of wild type (WT) or mutated proteins. (D) Percentages of embryos that show reduced placode expression of indicated genes after expression of wild type (WT) or mutated proteins. (E) Percentages of embryos that show reduced chd expression after expression of wild type (WT) or mutated proteins.
Image published in: Marchak A et al. (2017)
Copyright © 2017. Image reproduced with permission of the Publisher, Elsevier B. V.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| foxd3.L | fkh6, foxd3-a, foxd3-b, XFD-6, xfoxd3 | X. laevis | Throughout NF stage 18 | cranial neural crest |
| zic2.L | hpe5, xzic2, zic2-a, zic2-b, Zinc finger protein of the cerebellum 2 | X. laevis | Throughout NF stage 18 | neural plate neural plate border |
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