Fig 2. Test crosses between animals carrying tyr-mutated leapfrog transplants and albinos demonstrate germline transmission of mutant alleles. (A) Leapfrog transplant-bearing male (pigmented) is shown amplexed with an albino tyr−/− female. (B) Leapfrog transplant-bearing female (pigmented) is shown amplexed with an albino tyr−/− male. (C) Examples of F1 progeny from the cross in A grown to tadpole stage. These tadpoles are albino because they inherited tyr mutant alleles from both F0 parents. Therefore the leapfrog-generated frog carries gametes derived from CRISPR-mutated PGCs. The inset in C shows an unrelated pigmented tadpole at roughly the same stage for comparison.
Image published in: Blitz IL et al. (2016)
Copyright © 2016. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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