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Fig. 6 (left). Spatio-temporal expression of pou3f3 during Xenopus laevis development. (A) Expression of pou3f3 analyzed by real-time quantitative polymerase chain reaction (RT-qPCR) at indicated stages of development. (B) Whole mount in situ hybridization for pou3f3 at indicated stages of development. Neurula, dorsal view (a,b). Tailbud, dorsal (c) and lateral (d,f) view. Tadpole, lateral view (g,i). Transverse sections at the level of the developing pronephros (e,h). Abbreviations: (fb) forebrain, (hb) hindbrain, (mb) midbrain, (nt) neural tube, (ov) otic vesicle, (p) pronephros. (C) Expression of pou3f3 analyzed by RT-qPCR on dissected explants from tailbud-stage embryos (stage 25). Embryos were dissected either into ventral and dorsal halves or into tail bud, pronephros and head. **: P < 0,005; ***: P < 0,001, relative to the ventral half.

Image published in: Cosse-Etchepare C et al. (2018)

Copyright © 2018. Reproduced with permission of the Publisher, University of the Basque Country Press.

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