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Fig. 7 (right). Spatio-temporal expression of pou3f4 during Xenopus laevis development. (A) Expression of pou3f4 analyzed by real-time quantitative polymerase chain reaction (RT-qPCR) at indicated stages of development. Average values from three independent experiments. (B) Whole mount in situ hybridization for pou3f4 at indicated stages of development. Neurula, dorsal view (a). Tailbud, dorsal (b) and lateral view (c,e). Tadpole, lateral (f,i) view. Transverse sections at the level of the developing pronephros (d,h) and otic vesicle (g). Abbreviations: (fb) forebrain, (hb) hindbrain, (mb) midbrain, (nt) neural tube, (om) otic mesenchyme, (ov) otic vesicle, (p) pronephros. (C) Expression of pou3f4 analyzed by RT-qPCR on dissected explants from tailbud-stage embryos (stage 25). Embryos were dissected either into ventral and dorsal halves or into tail bud, pronephros and head. *: P < 0,05; **: P < 0,005, relative to the whole embryo.

Image published in: Cosse-Etchepare C et al. (2018)

Copyright © 2018. Reproduced with permission of the Publisher, University of the Basque Country Press.

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