Fig. 2.Temporal expression ofslc7a5.Sandslc7a5.Linthe developmental stages.Semi-quantitative RT-PCR was performed to examine the expression ofslc7a5 mRNA in the developmental stages.slc7a5.Sstarted to express from the gastrula stage(st.10.5) and increased thereafter.slc7a5.Lexpressionwas found maternally and also detected in the zygoticstage.slc3a2(4F2hcorCD98) expression was detected from st.20.Histone H4was used as a loading control.−RT represents a negative control without reverse transcriptase. Numbers show developmental stages de-fined by Nieuwkoop and Faber (1994). U: unfertilized egg.
Image published in: Katada T and Sakurai H (2019)
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