Figure 6. Premature ZGA in Miniature Embryos Rules out a Cell-Cycle Counter Model and Supports a Cell Sizer Model for ZGA. (A) Schematic predictions of ZGA onset in wild-type (WT) and miniature (mini) embryos for a counter and sizer model. (B) Representative sagittal slices of WT, midi (half volume, 1/2 V), and mini (quarter volume, 1/4 V) embryos stained for DNA (black pseudocolored). Scale bars, 100 μm. (C) Volume measurements for WT (gray), midi (orange), and mini (red) embryos at blastula stages. Each point indicates one embryo. Data represented as mean ± standard deviation. (D) Percentage of transcriptionally active cells as a function of embryonic cleavage for WT (gray), midi (orange), and mini (red) embryos. Each point indicates one embryo. Data pooled from four independent experiments. Exponential fits to data as visual aid. (E) Histogram of cell-size distributions for WT embryos at ∼2,000-cell stage (∼C11; gray) and ∼8,000-cell stage (∼C13; black) and mini-embryos at ∼2,000-cell stage (∼C11; red). Yellow stripe indicates a putative threshold of ∼45 μm. (F) Fraction of transcriptionally active cells as a function of cells smaller than 35 μm for WT (black) and midi and mini (red) embryos. Each square indicates one embryo. Linear fits to data. See also Figure S6.
Image published in: Chen H et al. (2019)
Copyright © 2019. Image reproduced with permission of the Publisher, Elsevier B. V.
Permanent Image Page
Printer Friendly View
XB-IMG-176002