Figure 7. Clk2 modulates BMP and FGF signals. (a) Clk2 decreases the level of p‐Smad1/5/8 in cooperation with BMP signal inhibition. clk2 SYA‐GR (250 pg and 500 pg) and δBMPR (135 pg) mRNAs were injected into the animal region of X. laevis embryos at the 4‐cell stage. At the blastula stage, embryos were transferred to a medium containing DEX and the ectodermal explants were isolated. At the mid‐gastrula stage (stage 11.5), whole cell extracts were immunoblotted with anti‐phospho Smad1/5/8 (p‐Smad1/5/8), anti‐Smad1 (Smad1), and anti‐tubulin antibodies. Tubulin was used as the loading control. (b) Clk2 activates MAPK together with FGF. clk2 SYA‐GR (250 pg and 500 pg) and efgf (10 pg) mRNAs were injected into the animal region of X. laevis embryos at the 4‐cell stage. At the blastula stage, embryos were transferred to a medium containing DEX and the ectodermal explants were isolated. At the early gastrula stage (stage 10), whole cell extracts were immunoblotted with anti‐diphosphorylated MAPK (dp‐MAPK), anti‐MAPK (MAPK), and anti‐tubulin antibodies. Tubulin was used as the loading control.
Image published in: Virgirinia RP et al. (2019)
Copyright © 2019. Image reproduced with permission of the Publisher, John Wiley & Sons.
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