FIGURE S1 | Expression patterns for WHS related genes across early development. In situ hybridization utilized (A–F) antisense mRNA probe to whsc1, (G–L) full-length antisense mRNA probe to whsc2, (M–R) full-length antisense mRNA probe to letm1, and (S-X) 1 kb partial-length antisense mRNA probe to tacc3. Embryos shown at blastula stage (A,G,M,S), in dorsal view at stage 16–20 (B,H,N,T), in lateral view at stage 20–25 (C,I,O,U), detail of lateral anterior region at stage 35 (D,J,P,V), and in both lateral and dorsal views from stages 39–42 (E,K,Q,W and F,L,R,X). (Y) In situ hybridization probes generated against sense strands of WHS gene mRNAs, shown at stage 25. Brown coloration (S,T) is unbleached pigment, unrelated to in situ hybridization staining. Scalebar is 250 μm.bryos were anesthetized with benzocaine.
Image published in: Mills A et al. (2019)
Copyright © 2019 Mills, Bearce, Cella, Kim, Selig, Lee and Lowery. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| nsd2.L | whsc1 | X. laevis | Throughout NF stage 9 | |
| nsd2.L | whsc1 | X. laevis | Throughout NF stage 16 | neural plate presumptive axial mesoderm |
| nsd2.L | whsc1 | X. laevis | Throughout NF stage 25 | cranial neural crest eye primordium neural tube |
| nsd2.L | whsc1 | X. laevis | Throughout NF stage 35 and 36 | otic vesicle cranial neural crest brain eye |
| nsd2.L | whsc1 | X. laevis | Throughout NF stage 41 | brain spinal cord head mesenchyme cardiac mesoderm hindbrain rhombomere |
| nelfa.L | LOC108706516, whsc2 | X. laevis | Throughout NF stage 20 | paraxial mesoderm neural tube |
| nelfa.L | LOC108706516, whsc2 | X. laevis | Throughout NF stage 25 | cranial neural crest brain eye presomitic mesoderm somite |
| nelfa.L | LOC108706516, whsc2 | X. laevis | Throughout NF stage 35 and 36 | cranial neural crest brain eye |
| nelfa.L | LOC108706516, whsc2 | X. laevis | Throughout NF stage 39 | spinal cord head brain migratory trunk neural crest cell |
| letm1.L | X. laevis | Sometime during NF stage 8 to NF stage 9 | embryo | |
| letm1.L | X. laevis | Sometime during NF stage 16 to NF stage 20 | embryo | |
| letm1.L | X. laevis | Throughout NF stage 35 and 36 | brain eye cranial neural crest hyoid crest anterior branchial crest posterior branchial crest intermediate mesoderm | |
| letm1.L | X. laevis | Throughout NF stage 39 | brain head intersomitic region spinal cord | |
| tacc3.L | eric1, maskin, xmaskin | X. laevis | Sometime during NF stage 8 to NF stage 9 | embryo |
| tacc3.L | eric1, maskin, xmaskin | X. laevis | Throughout NF stage 16 | neural plate neural plate border |
| tacc3.L | eric1, maskin, xmaskin | X. laevis | Throughout NF stage 25 | cranial neural crest brain eye head spinal cord |
| tacc3.L | eric1, maskin, xmaskin | X. laevis | Throughout NF stage 35 and 36 | brain eye head pharyngeal arch pronephric mesenchyme |
| tacc3.L | eric1, maskin, xmaskin | X. laevis | Throughout NF stage 42 | brain eye heart hindgut fin margin |
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