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Fig. 3. Expression of XSGLT1k examined by colorimetric in situ, FISH and FCIS methodologies. (A and B) XSGLT1k expression in stage 28/29 pronephros (red arrow). (C and D) XSGLT1k expression in stage 37 pronephros. (E) XSGLT1K expression in stage 37 embryo detected via FISH with an FITC-tyramide substrate. (F) Na+K+ATPase expression in the same embryo as panel F, detected with a Cy3-tyramide substrate. (G) Overlay of panels E and F. The proximal domain expressing XSGLT1k appears green. (H) XSGLT1k expression in a stage 30 embryo developed with a purple substrate. (I) The same embryo as shown in panel H but developed for Na+K+ATPase expression with an FITC-tyramide substrate. (J) FCIS overlay of panels H and I performed via the protocol described in Methods. The XSGLT1k domain appears blue, while the pronephric tubules in which it is not expressed appear orange.

Image published in: Zhou X and Vize PD (2004)

Copyright © 2004. Image reproduced with permission of the Publisher, Elsevier B. V.

GeneSynonymsSpeciesStage(s)Tissue
slc5a9.Lsglt1k, sglt4, XSGLT1KX. laevisThroughout NF stage 28pronephric kidney
pronephric mesenchyme
slc5a9.Lsglt1k, sglt4, XSGLT1KX. laevisThroughout NF stage 37 and 38early proximal tubule
pronephric kidney
late proximal tubule
atp1a1.Latp1a1-a, atp1a1-b, K-ATPase, Na+/K+ ATPase, Na+K+ ATPase, Na+K+ATPaseX. laevisThroughout NF stage 29 and 30pronephric kidney
pronephric duct
pronephric tubule

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