Figure 6. Analysis of marker gene expressions in stage 10.5 embryos injected with 14-3-3 tau or epsi morpholinos. a-g: 14-3-3 tau and epsi morpholinos injections blocked Xbra expression. a,e: Two-cell stage embryos were injected into both blastomeres with a total of 10 pmol 14-3-3 tau - or epsi -specific morpholinos. Xbra expression is greatly reduced around the marginal zone, which is normally stained in control morpholino-injected embryos (d). b,f: Two-cell stage embryos were injected unilaterally with 10 pmol of 14-3-3 tau - or epsi -specific morpholinos. Note the reduced Xbra expression on the injected side. c,g: Two opposite blastomeres of four-cell stage embryos were injected with a total of 10 pmol of 14-3-3 tau - or epsi -specific morpholinos. Xbra expression is deficient in two of the four quadrants. h-j: Two-cell stage embryos were injected bilaterally with 10 pmol of 14-3-3 tau -specific (h), 14-3-3 epsi -specific (i), or control (j) morpholinos. Embryos were fixed at stage 10.5 and subjected to in situ hybridization with chordin probe. Embryos injected with 14-3-3 tau or epsi morpholinos (h,i) had the same chordin expression as control-injected embryos (j).
Image published in: Lau JM et al. (2006)
Copyright © 2006. Image reproduced with permission of the Publisher, John Wiley & Sons.
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